Craniosynostosis involves premature or disordered suture closure resulting in skull asymmetry. This condition occurs in approximately 1 in 2500 births, and most are inherited from an affected parent or occur as a de novo autosomal dominant mutation. However, a few of these conditions result from autosomal recessive inheritance including Antley-Bixler, Carpenter, and Baller-Gerold syndromes. Craniosynostosis syndromes exhibit genetic heterogeneity as well as phenotypic variability with features that may include facial dysmorphism, palatal abnormalities, short stature, proptosis, and developmental delays. Craniosynostosis may also occur as an isolated finding. The 8 genes included on this panel account for multiple distinct clinical phenotypes.